Quantification of carbohydrates in various matrices

The quantification of carbohydrates can be challenging due to their high polarity, low
volatility, their lack of a chromophore and their common occurrence in complex matrices.
HPTLC separates mono- and oligosaccharides after minimal sample preparation and
sensitively detects and quantifies these compounds after post-chromatographic
derivatization. With the two methods developed for the HPTLC PRO System described in
this application note, many samples can be rapidly analyzed at low running costs. The
methods are suitable for the analysis and quantification of carbohydrates in various
matrices like syrups, honeys, and molasses.

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