How big is too big for chromatography?

Utilizing pore volume and shear force for molecular separation

Gene therapeutic advancements continue to challenge the limits of traditional sizing techniques.

In this series, the Waters Research & Development team explores innovations in wide-pore size-exclusion chromatography (SEC) and introduces a groundbreaking new approach: slalom chromatography (SC).

Together, these techniques offer advanced solutions for sizing and characterizing complex modalities including lipid nanoparticles (LNPs), lentiviral vectors (LVVs), and nucleic acid constructs.

Learning Objectives:

• Learn how to attain accurate size profiles for viral vectors, plasmids, and LNPs with SEC with improved recovery
• Understand how to quickly implement your SEC-MALS methods and cut through the noise
• Revisit slalom chromatography as a tool for separating double-stranded nucleic acids
• Explore how slalom chromatography can provide a fast alternative to gels and ELISA for plasmid DNA and dsRNA quantitation

Join us on our journey to unlock clearer insights into the largest and most intricate biomolecules in development today.

Additional resources

• Efficient Profiling of Lipid Nanoparticle Formulations Using Waters GTxResolve 2000 Å SEC Column, MaxPeak Premier 3 µm
• Retention mechanism in combined hydrodynamic and slalom chromatography for analyzing large nucleic acid biopolymers relevant to cell and gene therapies

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